i would say that it isn't good to pick her up, as reassuring as it may be for her. if it is indeed sep anxiety - you need to stay in the room until she falls asleep ... in her crib. i usually just put and hand on my lo's belly if she's having this problem and stay there until she is fast asleep, as this way i am not reinforcing her waking (not making it too appealing for her to wake) but am still reassuring her that i am there. if this does not work for your lo you may want to try pu/pd if she is crying and you can't settle in the crib. the middle of the night can be a very unappealing time to start pu/pd so if you are thinking it make sure you are prepared and be consistant b/c if you aren't then your lo will be confused and it won't work. it make take anywhere from a few days to weeks but the results are worth it in the end.