I would say 1 hour after nursing, if yo uare consistent with pumping, you will produce more, say every day after the 9 am feeed you pump at 10 am - your body will adjust and create more of a supply for that timeframe. also if you are looking to increase supply amount, try mothers milk tea. it is fantastic, deink it for two days, and see a difference. also remember to load up on the water, especially wehn pumping more, it will help with the supply.